In this thesis, i present a theory for charged nanocolloid dielectrophoresis taking into account the surface charge and debye double layer effects. To delete one page from a pdf you dont need to download or install any software. In 1992, huang established the model of traveling wave dielectrophoresis. Dielectrophoresis on nanostructured substrates for. Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue. March 18, 2002 electrostatic fields have two observable effects upon the hydrostatics of liquids. All particles exhibit dielectrophoretic activity in the presence of electric fields.
Dielectrophoresis can discriminate distinct cellular identities in heterogeneous populations, and monitor cell state changes associated with activation and clonal expansion, apoptosis, and necrosis, without the need for biochemical labels. Features the relevant chemical and biological concepts for those working in physics and engineering. This thesis presents models and simulations of dielectrophoretic separation of micro and. Pdf this thesis presents basic experimental studies in soft and complex. Pdf on the dielectrophoretic particle retention in. Piezoelectric composites structured via dielectrophoresis. In recent years, dielectrophoresis has developed rapidly and its presentday field of application is broad and diverse both in inanimate and in biological systems. The dep force exerted on the cell is given by 2 3 2. Dielectrophoresis and microfluidics research papers.
Dielectrophoresis article about dielectrophoresis by the. We can imagine that, in the future, a smartphone can work in conjunction with a piece of hardware that can sample and analyze blood. Replace the attched file with the revised file and resubmit. On the relationship of dielectrophoresis and electrowetting. Versatile dielectrophoresis based microfluidic platforms. Cell manipulation and separation has numerous biological and medical applications, e. Insulatorbased dielectrophoretic manipulation of dna in a microfluidic device by lin gan a dissertation presented in partial fulfillment of the requirements for the degree doctor of philosophy approved july 2015 by the graduate supervisory committee. Among active singlecell trapping schemes, dielectrophoresis is the most commonly used method, which confines cells via their inducible electric dipoles in an electric field gradient, featured with selective cellular capture and release 1416. Doctoral thesis, nanyang technological university, singapore. Electrophoresis, as its name suggests, measures the electric properties of a material that is, its net charge. Click a category and then select a filter for your results.
The removal of chargeneutral particles by the principle of. Dielectrophoresis and its applications in biochips. We investigate the dielectrophoretic separation of microparticles. The force f on a charge is f qe, so if the net charge is zero, there is no force. V we use cookies to help provide and enhance our service and tailor content.
Solutions for cardiopulmonary organ repair and regeneration scorr scientific research day, march 2017. Alexandra ros, chair daniel buttry yan liu arizona state university august 2015. Limit your results use the links below to filter your search results. A travelingwave electric field will be produced if the phaseshifted voltages are applied to the parallel electrodes. We later take advantage of the fact that no such restriction need be imposed for.
Dielectrophoresis and its application to biomedical diagnostics platforms. In this work were produced two composites via dielectrophoresis, for two different fields of application. Modeling of dielectrophoresis in micro and nano systems. Delete pages from pdf remove pages from documents for. Figure 1 illustrates the behaviors of particles in the nonuniform electric field. A method was developed for studying the dielectrophoretic properties of both homogeneous and mixed populations of mammalian cells. Dielectrophoresis is defined as the motion of a neutral particle caused by polarization effects in a nonuniform electric field1,2. Piezoelectric composites structured via dielectrophoresis for. A dipole has net charge 0 and will experience a torque in an electric field, but no translational force. It uses a dc voltage to attract anions and cations to the relevant counterelectrode, and the speed and direction of moveme. Information and translations of dielectrophoresis in the most comprehensive dictionary definitions resource on the web. Dep is a phenomenon related to electrophoresis, but with some important differences.
View dielectrophoresis and microfluidics research papers on academia. This thesis is brought to you for free and open access by the theses at. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. Thesis submitted to the faculty of graduate and postdoctoral studies.
An array of circular dielectrophoresis traps to separate. Dielectrophoresis dep is a phenomenon in which a force is exerted on a dielectric particle when it is subjected to a nonuniform electric field. Dielectrophoresis and its application to biomedical. It is important to then rinse the sample in di water and dry it thoroughly before the final.
Iso dielectrophoresis ids can be used to characterize and separate cells by their electrical characteristics. Dielectrophoresis, the movement of particles in nonuniform ac electric field, is of. I have a question regarding the solver while solving the dielectrophoresis. This thesis is based on studies in which computer simulations were key elements in the development of several new tools for use in gas chromatography and dielectrophoresis. It is an electrostatic phenomenon, distantly related to electrophoresis, and describes the motion of suspended particles resulting from polarisation forces produced by an inhomogeneous electric field. Dielectrophoresis or dep is an important phenomenon which is induced when a dielectric particle is placed in a nonuniform electric field. Theoretical study of spermatozoa sorting by dielectrophoresis or magnetophoresis with supervised learning.
Pdf dielectrophoretic separation of mammalian cells. This file contains additional information such as exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. Electrical engineering, massachusetts institute of technology, 2014 submitted to the department of electrical engineering and computer science in partial fulfillment of the requirements for the degree of masters of engineering in electrical engineering and computer science at the. Dielectrophoresis is the electrokinetic motion of particles that occurs when a polarizable particle is placed in nonuniform electric fields, and the particle motion is influenced by the ambient electric field and by the properties of the dielectric particles or solutions lee et al. As an ideal method to manipulate biological particles, the dielectrophoresis dep technique has been widely used in clinical diagnosis, disease treatment, drug development, immunoassays, cell sorting, etc. Thesis, massachusetts institute of technology, cambridge, ma, usa, june 2001.
In the 1950s, pohl 1951, 1958 described dielectrophoresis as the movement of neutral particles under the influence of nonuniform electric fields. Pdf on the dielectrophoretic particle retention in porous media. This force does not require the particle to be charged. Dielectrophoretic traps have been broadly studied in light of their many advantages of high controllability, ease of operation, and high efficiency. In gas chromatography, gaseous analytes are separated by exploiting differences in their partitioning between different phases, and after their partitioning parame. Computerized image analysis was employed to quantify the rate of. When the dielectric constant of particle is larger than that of. Polarization and manipulation at liquidliquid interfaces by mitchell desmond a thesis submitted to johns hopkins university in conformity with the requirements for the degree of master of science engineering baltimore, maryland august, 2014. The nonuniform electric field is formed by applying ac voltage between a pin electrode and a plate electrode. As before, assume that the nonuniformity of this field is modest on the scale of the particles dimensions. Jan 23, 2015 dielectrophoretic separation of particles. Dielectrophoresis and its applications in biochips by. Electrophoresis, as its name suggests, measures the electric properties of a.
The dielectrophoretic motion is determined by the magnitude and polarity of the charges induced in a particle by the applied field 8. Dielectrophoresis of small particles eprints soton. Insulatorbased dielectrophoretic manipulation of dna in a. Dielectrophoresis and its applications in biochips by haibo li. Dielectrophoresis dep is a versatile technique for the solution of difficult bioparticle separation tasks based on size and material. In order to exploit idep for naturally formed dna and dna nanoassemblies, a detailed understanding of the underlying polarization and. Aug 29, 2017 this file contains additional information such as exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. An ids system usually consist of a rf frequency voltage source up to 24 v, syringe pumps, microscope, and computer. Payen university of california, berkeley bio and thermal engineering laboratory eecs 245 abstract basic ic fabrication techniques are employed in this paper with the purpose of designing a micro. The removal of chargeneutral particles by the principle of dielectrophoresis electrophoresis and dielectrophoresis electrophoresis, the phenomenon of a charged particle being drawn to an oppositely charged surface, is fairly easy and intuitive to understand. Graduate thesis or dissertation integration of vapor. Biological cell separation using dielectrophoresis in a microfluidic device r.
Dielectrophoresis an overview sciencedirect topics. The use of microfluidics and dielectrophoresis for separation. Consider the concentric, dielectric shell subjected to an electric field e o in figure 2a. The emphasis is on simulating directcurrent electrokinetic phenomena such as electroosmosis, electrophoresis, dielectrophoresis, and inducedcharge electrokinetics. Furthermore, by shrinking the gap between gold electrodes to sub10 nm, we show ultralowpower trapping of nanoparticles and biomolecules. Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest. The uid dynamics involved and the dielectric properties of water inside singlewalled carbon nanotube are studied as well. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. As an example of the growing importance of dielectrophoresis, two special issues focused on dielectrophoresis were published by the journal electrophoresis in september 2011 2, 3. This video is for the 2018 science ambassador scholarship. Ieee industry applications society for presentation annual meeting, united states.
Kth, school of engineering sciences sci, mechanics. In the thesis, an array of circular dielectrophoresis dep traps was developed and tested to manipulate population of. This thesis focus in dielectrophoretic approach due. Dielectrophoresis in thin liquid films eindhoven university. Portable iso dielectrophoresis system by lisa liu b. Modeling of dielectrophoresis in micro and nano systems yuan lin 2008 kth mechanics se100 44 stockholm, sweden abstract this thesis presents models and simulations of dielectrophoretic separation of micro and nano particles. This phd thesis is the result of a collaboration between two laboratories.
Dielectrophoresis for bioparticle manipulation mdpi. The force generated by dep has been exploited for various micro and nano fluidics applications like positioning, sorting and separation of particles involved in medical diagnostics, drug discovery, cell therapeutics, biosensors, microfluidics. The content presented in this document is the product of original work performed by the author at. Dielectrophoresis dep is the movement of dielectric particles in an inhomogeneous electric field. Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying dep to unmet needs in such areas as. However, the strength of the force depends strongly on. Development of microsystems for the controlled formation of cell. Dielectrophoresis on nanostructured substrates for enhanced. Lets envision a case where this type of analysis can be divided into three steps. Graduate thesis or dissertation from electrophoresis to. Simply upload your file, delete pages from your pdf file and download it again. Dielectrophoresis often abbreviated dep was first observed early in the 20th century, but described fully and named herbert pohl in 1950.
Theoretical study of spermatozoa sorting by dielectrophoresis. In the previous studies, however, it was challenging to count captured particles or required work to capture particles. Electrophoresis 1 trapping of dna by dielectrophoresis 2. An array of circular dielectrophoresis traps to separate and.
Depending on the electric properties of the particles, they can. Isodielectrophoresis ids can be used to characterize and separate cells by their electrical characteristics. Medical analysis and diagnostics on smartphones is about to undergo rapid growth. The aes electrophoresis society is pleased to launch the premier online resource for dielectrophoresis and the multitude of specialty subtopics.
Insulatorbased dielectrophoresis idep provides an efficient and matrixfree approach for manipulation of microand nanometersized objects. Assisted reproduction currently accounts for over one in every hundred births in developed countries. Delete each page by hovering over its thumbnail and click the trash icon. Electric field structuring is an easier way to produce composites, exploiting a phenomenon called dielectrophoresis. Dielectrophoresis dep is a separation method in which a nonuniform. Pdf particle assembly guided by electrohydrodynamics and. The exact mechanism of the origin of the stern layer, through the surface conductance effect of a nanocolloid to form. Integration of vaporsolid grown zno nanowires through dielectrophoresis. A transparent electrode is integrated with the plasmonic substrates that allow dielectrophoresis without interfering with the labelfree sensing schemes such as surface plasmon resonance or raman spectroscopy. A study based on comsol multiphysics modeling a thesis presented to the graduate school of clemson university in partial fulfillment of the requirements for the degree master of science bioengineering by vandana devi pandian babu august 20 accepted by. The particle subjected to the travelingwave field will move along or against the direction of filed travel. Versatile dielectrophoresis based microfluidic platforms for chemical stimulation of cells tang, s 2015, versatile dielectrophoresis based microfluidic platforms for chemical stimulation of cells, doctor of philosophy phd, electrical and computing engineering, rmit university.
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